TABLE 2.

Kinetics of reduction of CYPOR by 1 and 10 molar equivalents of NADPH at 30 °C

See under “Experimental Procedures” for experimental details. k represents the rate constant, and A represents the phase amplitude.

Reductase	λ nm	A1	k1	A2	k2	A3	k3
		%	s−1	%	s−1	%	s−1
1 Equivalent
Wild type	452	79	75	21	6.1
	585	62	79	38	6.2
147CC514	452	25	64	43	12	32	0.8
	585	39	1.7	61	0.2
10 Equivalents
Wild type	452	73	76	27	9.1
	585		91		11 (decay)a
147CC514	452	43	52	17	2.5	40	0.7
	585		2.4		0.02 (decay)a

^a Data represent a decrease in absorbance due to formation of the four electron-reduced CYPOR.