TABLE 5.

Activity of wild type and 147CC514 CYPOR with cyt c and P450 before and after treatment with DTT alone or with DTT and iodoacetamide

The experiments were performed as discussed under “Experiment Procedures.” The DTT and iodoacetamide (IAM) were removed from the 147CC514 protein prior to the test of its activity.

Protein	Nanomoles of cyt c reduced per s/nmol CYPOR			Nanomoles of CH2O produced per s/nmol P450-CYPOR
	−DTT	+DTT	+DTT/IAM	−DTT	+DTT	+DTT/IAM
Wild type CYPOR	62 ± 6.2	68 ± 5.0	62 ± 4.5	0.84 ± 0.11	0.88 ± 0.11	0.83 ± 0.13
Full-length 147CC514	2.31 ± 0.35	60 ± 4.5	54 ± 6.0	0.09 ± 0.01	0.50 ± 0.09	0.57 ± 0.08