FIGURE 2.

Demonstration of a molecular association between Rac2 and PLD2. A, Myc- and HA-tagged constructs are shown. COS-7 cells were transfected with either Myc-Rac2 or HA-PLD2 alone or in combination. After 2 days, cells were incubated in the absence of EGF, and lysates were immunoprecipitated (I.P.) with the indicated antibodies. Resulting Western blots (W.B.) were probed with the indicated antibodies (B). C, fluorescent (YFP and CFP)-tagged constructs were transfected in COS-7 and analyzed as previously described by immunoprecipitation and WB (D). The bottom row of each panel is a control that shows equal loading in all lanes as ascertained with anti-actin antibodies.