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Deletion of the unstructured loop 1115–1138 does not alter nucleotide binding characteristics of the N-domain. ATP binding to the wild-type N-domain (open squares), Glu1064-WNDΔ1115–1138 (triangles), and Ala1064-WNDΔ1115–1138 (diamonds) was monitored by measuring compound chemical shift change of the backbone amide group of Gly1101.
