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. 2011 Mar 18;286(18):16435–16446. doi: 10.1074/jbc.M110.194928

FIGURE 4.

FIGURE 4.

MBNL1 splicing function requires the region encoded by MBNL1 exon 6. A, truncated MBNL1 constructs were transiently expressed in HeLa cells. The name of each construct is indicated at the top. The regions expressed are indicated, and the presence of alternative exons is shown in subscripts. The level of expression of the different isoforms was verified to be the same by Western blotting using an antibody raised against the GFP tag. GAPDH was used as a loading control. B and C, truncated MBNL1 constructs were co-expressed in HeLa cells in the presence of hcTNT exon 5 (B) or IR exon 11 (C) minigenes. The name of each construct is indicated at the top and the regions expressed with the presence of alternative exons in subscripts. Cells were lysed 48 h after transfection, and splicing products were assayed by RT-PCR. Data are expressed as indicated for Fig. 3. An unpaired t test with two tails was used to compare values. Statistically significant results are indicated by * (p < 0.05), ** (p < 0.001), and *** (p < 0.0001). Error bars, S.E.