FIGURE 4.

Thermodynamics of the binding of C1q to IgG1 and CRP. Changes in the thermodynamic parameters, enthalpy (ΔH), entropy (TΔS), and Gibbs free energy (ΔG), during the association, dissociation, and equilibrium phases of the binding of native (white bars) and hemin-exposed (black bars) C1q to IgG1 (A), monomeric CRP (B), and pentameric CRP (C). Reported values were obtained by applying thermodynamic calculations (see “Experimental Procedures”) to the Arrhenius plots shown in Fig. 3. The thermodynamic parameters were determined by using a reference temperature of 298 K (25 °C).