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. 2011 Mar 18;286(18):16504–16515. doi: 10.1074/jbc.M110.208447

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Dose-dependent effects of MB in mitochondrial complex I-III and II-III activity in mitochondrial extracts. A, representative assay of complex I-III activity; B, complex I-III activity with increasing dose of MB or acetyl MB, which is redox-disabled; C, chemical structure of MB, MBH₂, and acetyle MB. D, complex I-III activity with increasing dose of MB with/without the presence of complex I inhibitor (rotenone). E, complex I-III activity with increasing dose of MB with/without the presence of complex III inhibitor (antimycin A). F, complex II-III activity with increasing dose of MB with/without the presence of complex III inhibitor (antimycin A). *, **, and ***, significant difference from those without antimycin. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Error bars, S.E.