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. 2011 Mar 18;286(18):16504–16515. doi: 10.1074/jbc.M110.208447

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — MB forms a redox cycle between complex I and III. A and C, MB is a complex I substrate accepting electrons from NADH in a dose-dependent manner and is insensitive to rotenone inhibition. Activity with coenzyme Q1 was used as 100% control. **, significant difference between the indicated groups. B and D, reduced MB donates electrons to oxidized cyt c in a dose-dependent manner. ***, significant difference from the MB-only group. ROT, rotenone; CoQ1, coenzyme Q1. Top panels, representative assays (A and B). Bottom panels, quantitative analysis of the corresponding assay (C and D). Error bars, S.E.