FIGURE 8.

MB attenuates rotenone induced fluoro-jade B-labeled neurodegeneration in TH-positive dopaminergic neurons. A, many degenerated cell bodies of nigrostriatal dopaminergic neurons were positively labeled by fluoro-jade B in the SNC area in ROT/Sal animals. B, nigral neurons were double labeled with fluoro-Jade B and TH, showing that these were degenerating dopaminergic neurons in SNC. The arrowheads indicate the presence of double labeled neurons with both TH and fluoro-jade B. Such fluoro-jade B-positive neurons were identified in half of the ROT/Sal group that had severe neuronal loss and behavioral deficiency. No fluoro-jade B-positive neurons were detected in Veh/Sal or ROT/MB groups. Scale bar, 100 μm (A) and 50 μm (B).