FIGURE 5.

Assaying Co²⁺ transport activity of TmCorA (ΔE. coli + TmCorA) by monitoring the loss of ΔE. coli growth activity caused by Co²⁺ toxicity. ΔE. coli lacking any functional CorA shows resistance toward Co²⁺ by retaining its growth activity. The presented data points are the average of three different measurements as described under “Experimental Procedures.”