FIGURE 7.

Assaying Mg²⁺ transport activity of CorA homologues (A–C). The strain MM1278, a derivate of MM281 that expresses StCorA and can completely restore the growth activity of the strain, was used as positive control. pBAD indicates MM281 strains transformed with the empty pBAD vector. TmCorA and EcCorA are MM281 expressing TmCorA and EcCorA, respectively. The presented data points are the average of three independent experiments. The concentration of supplementary Mg²⁺ in the LB medium is indicated. D, the expression levels of TmCorA and EcCorA at different time points were analyzed by Western blotting, e.g. Tm4h indicates the expression level of TmCorA after 4 h.