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Mouse-human z-scores of motifs with experimentally verified ESS activity, which is deleterious for exons. Four of four of the Wang et al. [24] experimentally verified ESSs have negative z-scores, consistent with negative selection acting to remove them from coding regions. For the Zhang and Chasin [23] experimentally verified ESSs, nine of ten have negative z-scores (GTATTGTT, z = -0.006).
