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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Hypertension. 2011 Feb 14;57(4):859–864. doi: 10.1161/HYPERTENSIONAHA.110.167957

Figure 3.

Figure 3

Evidence of functional s(P)RR in the urine of Ang II hypertensive rats A. Detection of the soluble form of the (P)RR (28 kDa) in urine samples of sham-operated and Ang II infused rats. Eighty micrograms of protein from 10X concentrated urine samples were loaded and incubated with a rabbit anti-(P)RR. B. Immunoprecipitation of (P)RR in the urine samples of Ang II-infused and sham-operated rats demonstrated the absence of renin in the supernatant (S) fractions using the rabbit polyclonal anti-renin H-105 antibody (Santa Cruz, Cat ID sc-22752). C. A dose-response curve of hPR added in the experiment. D. Urines; initially collected with or without protease inhibitor cocktail, measured in the presence and absence of 10 μl of hPR (2.2 pmol/μl), resulted in increased amounts of Ang I forming-enzymatic units excreted per day × 10−6 in Ang II-infused rats compared with sham-operated rats. In samples spiked with hPR, the background renin and non-specific enzyme activity were subtracted. MWM: molecular weight marker; IP: immunoprecipitate; WB: Western blot; hR: recombinant human renin; hPR: recombinant human (pro)renin.