Table 1.
Immunophenotypic characteristics of nonadherent, GM-CSF stimulated mouse liver-derived DC progenitors harvested from liquid cultures: comparison with GM-CSF stimulated spleen-derived DC
| Antigen (mAb) | Liver DC progenitors |
Spleen- derived DC |
|---|---|---|
| Leukocyte-common Ag | ||
| CD45 | ++ | ++ |
| CD45RA;B220 | − | − |
| MHC Class II; I-Ek,d,p,r | −/dim | +++ |
| DC-restricted | ||
| Lymphoid DC (33D1) | + | + |
| Interdigitating cell (NLDC 145) | + | + |
| Myeloid (primarily) macrophage (F4/80) |
+ | + |
| Lymphoid (primarily) | ||
| CD3∈ | − | − |
| Heat-stable antigen (J11D) | ++ | ++ |
| NK cells: NK1.1 | − | − |
| Receptors/adhesins | ||
| CD32, FcγRII | + | + |
| CD11b, MAC-1α unit; C3biR | ++ | + |
| CD11c, p150/90 (N418) | + | NDa |
| CD44, Pgp-1 | ++ | ++ |
| CD54, ICAM-1 | ++ | ++ |
| CTLA-4 counter-receptors | − | ++ |
| CD25, p55; IL-2R | − | − |
a
Not determined.