Sequential changes of L21-6+ cells in tissue sections of LEW heart allografts and BN recipient spleens after transplantation into (A) naïve animals without immunosuppression (group 2), (B) under continuous-TAC (group 4), (C) BMC-primed (group 5), and (D) liver transplant (Tx)-primed (group 6) recipients. For group 6, data are included from the LEW liver allografts that had been used for priming and been in place for 100 days by the time of challenge heart transplantation. After immunohistochemical staining, the number of L21-6+ cells was determined in 20 HPFs at original magnification × 400. Data from 3 to 6 animals at each time point are expressed as mean ± SD. (POD, postoperative day.)