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. Author manuscript; available in PMC: 2011 May 10.
Published in final edited form as: Liver Transpl. 2000 Nov;6(6):686–702. doi: 10.1053/jlts.2000.19029

Figure 6.

Figure 6

Double immunohistochemical stain of LEW MHC class II and CD86 with L21-6 mAb (3-amino-9-ethylcarbazole; red) and 24F mAb (blue alkaline phosphatase substrate kit IV; blue) in heart allografts 5 days after transplantation. (A) Untreated naïve BN recipient of group 2. Note many CD86+ cells associated with disappearance of L21-6+ cells and the upregulation of MHC class II on the microvasculature. (B) Heart allograft of group 6 in liver-primed recipient. Note double-positive purple donor cells (L21-6+, CD86+; arrow) among many single-stained L21-6+ cells but almost no single-stained CD86+ cells in the interstitium of the heart graft, i.e., a few MHC class II+ donor cells with upregulated B7-2 but essentially no recipient cells because the recipient was pretolerized to the donor. (Original magnification ×200.)