Table 2. Differentially Synthesized Proteins of A. fumigatus under Hypoxia in Comparison to Normoxic Conditions in the Mitochondrial Fraction.
| spot no.a | putative function and protein name (AFUA_...)b | ratioc |
|---|---|---|
| Amino acid metabolism | ||
| Aspartate | ||
| 1 | Aspartate aminotransferase (4G10410)d | 2.17 |
| Glutamate | ||
| 2 | Glutamate/Leucine/Phenylalanine/ Valine-DH (4G06620) | 1.95 |
| Lysine | ||
| 3 | Homoaconitase LysF (5G08890)d,e | 2.14 |
| Valine/Leucine/Isoleucine | ||
| 4 | Dihydroxy acid dehydratase Ilv3 (2G14210)d,e | 2.11 |
| 5 | Mitochondrial acetolactate synthase small subunit (4G07210)d,e | 1.62 |
| Protein folding | ||
| 6 | Mitochondrial Hsp70 chaperone (2G09960)d | 2.13 |
| 7 | Hsp70 chaperone (HscA) (8G03930)e | 1.63 |
| 8 | Antigenic mitochondrial protein HSP60 (2G09290)d,e | 1.77 |
| 9 | Calnexin (4G12850)e | −2.57 |
| Protein degradation | ||
| 10 | Aspartic endopeptidase Pep2 (3G11400)e | 2.32 |
| Secondary metabolism | ||
| 11 | Alpha/beta hydrolase (8G00530) | 4.51 |
| 12 | Methyltransferase SirN-like (8G00550) | 3.79 |
| Cytoskeleton/Septum-associated structure | ||
| 13 | Woronin body protein HexA (5G08830) | 5.68 |
| 14 | Septin AspA (5G08540)e | −1.86 |
| 15 | Septin (5G03080) | −1.54 |
| Fatty acid metabolism | ||
| 16 | Lysophospholipase Plb3 (3G14680)e | 11.2 |
| 17 | Lysophospholipase Plb1 (4G08720)e | 5.48 |
| 18 | Long chain fatty alcohol oxidase (1G17110)e | 1.7 |
| 19 | Acetyl-coenzyme A synthetase (4G11080) | −4.92 |
| 20 | Mitochondrial glycerol-3-phosphate DH (1G08810)d,e | −1.69 |
| Pentose phosphate pathway | ||
| 21 | 6-Phosphogluconate-DH Gnd1 (6G08050) | 1.9 |
| 22 | Transaldolase (5G09230) | 2.09 |
| Glycolysis/Pyruvate metabolism | ||
| 23 | Phosphoglycerate kinase PgkA (1G10350) | 1.51 |
| 24 | Enolase/allergen Asp F 22 (6G06770)e | 1.67 |
| 25 | Fructose-bisphosphate aldolase, class II (3G11690)e | 1.61 |
| 26 | Pyruvate-DH complex component Pdx1 (3G08270)d,e | 3.72 |
| 27 | Pyruvate-DH E1 component alpha subunit (1G06960) | 1.67 |
| TCA cycle | ||
| 28 | Aconitate hydratase, mitochondrial (6G12930)d,e | 2.41 |
| 29 | Succinyl-CoA synthetase alpha subunit (5G06130)d,e | 1.69 |
| 30 | Succinate dehydrogenase subunit Sdh1 (3G07810)d,e | −1.74 |
| Electron transport and oxidative phosphorylation | ||
| 31 | ATP synthase D chain, mitochondrial (6G03810)d,e | 3.02 |
| 32 | ATP synthase F1, beta subunit (5G10550)d,e | 1.98 |
| 33 | Mitochondrial F1 ATPase subunit alpha (8G05320)d | 1.85 |
| 34 | Ubiquinol-cytochrome C reductase complex core protein 2 (5G04210)d,e | 1.85 |
| 35 | Vacuolar ATP synthase catalytic subunit A (5G02370)e | 1.54 |
| 36 | Mitochondrial ATPase subunit ATP4 (8G05440)d,e | −2.02 |
| 37 | Electron transfer flavoprotein-ubiquinone oxidoreductase (3G10110)d,e | −1.64 |
| Ubiquitone biosynthesis | ||
| 38 | Ubiquinone biosynthesis monooxgenase (Coq6) (4G12930)d,e | 2.84 |
| 39 | Ubiquinone biosynthesis methlytransferase Coq5 (6G08850)d,e | 2.26 |
| Vitamin and cofactor biosynthesis | ||
| 40 | Thiamine biosynthesis protein (Nmt1) (5G02470) | 5.61 |
| 41 | Thiazole biosynthesis enzyme (6G08360) | 4.65 |
| Unclassified and proteins of unknown function | ||
| 42 | Protein of unknown function (6G11850)e | 34.4 |
| 43 | Protein of unknown function (3G08440)e | 1.78 |
| 44 | 1,3-beta-glucanosyltransferase Gel2 (6G11390)e | 7.35 |
| 45 | 1,3-beta-glucanosyltransferase (2G05340)e | 7.31 |
| 46 | 1,3-beta-glucanosyltransferase Gel1 (2G01170)e | 3.54 |
| 47 | GPI-anchored cell wall organization protein Ecm33 (4G06820)e | 5.78 |
| 48 | Outer mitochondrial membrane protein porin (4G06910)e | 4.26 |
| 49 | Stomatin family protein (3G13440)e | 3.14 |
| 50 | Ras GTPase Rab11 (1G02190)e | −1.74 |
a
Spot number in Figure 2.
b
DH, dehydrogenase.
c
Average ratios extracted from statistical analysis of DIGE gels by the Decyder software programs DIA and BVA.
d
Mitochondrial import sequence according to MitoProtII - v1.101.
e
Only found in the mitochondrial fraction as differentially regulated.