Table 3.
Comparison of fold changes between microarray and qRT-PCR
| Gene Accession # | Gene Symbol | 1 Day | 3 Day | 5 Day | 7 Day | ||||
|---|---|---|---|---|---|---|---|---|---|
| Micro-array | RT-PCR | Micro-array | RT-PCR | Micro-array | RT-PCR | Micro-array | RT-PCR | ||
| AJ721110 | VNN2 | 0.5 | 0.3 | 3.3 | 3.5 | 6.5 | 8.7 | 2.5 | 2.6 |
| AF070478 | MMP13 | 0.7 | 0.8 | 3.4 | 3.7 | 2.9 | 3.8 | 0.7 | 0.5 |
| AF062392 | MMP27 | 0.8 | 0.8 | 4.1 | 7.8 | 6.6 | 23.7 | 3.5 | 7.9 |
| X03509 | CKB | 0.9 | 1.4 | 4.7 | 6.8 | 5.5 | 11.0 | 2.0 | 3.3 |
| AJ309540 | IL6 | 0.9 | 1.1 | 4.8 | 7.7 | 7.2 | 37.1 | 3.1 | 5.7 |
| AF432506 | FABP4 | 1.0 | 1.2 | 5.3 | 7.8 | 7.1 | 33.4 | 15.0 | 10.0 |
| X65459 | FABP7 | 1.1 | 1.5 | 0.3 | 0.8 | 0.8 | 1.1 | 0.2 | 0.3 |
| AJ851480 | ACSL1 | 1.2 | 0.9 | 7.3 | 6.0 | 8.7 | 8.0 | 7.3 | 7.6 |
| AJ720861 | LSS | 1.3 | 1.1 | 1.1 | 0.7 | 1.3 | 1.1 | 0.2 | 0.1 |
| AJ719295 | Insulin induced gene 1 | 1.5 | 1.3 | 1.0 | 0.7 | 0.9 | 0.9 | 0.2 | 0.1 |
| AJ719718 | SC4MOL | 1.1 | 1.7 | 0.8 | 0.9 | 1.1 | 1.3 | 0.2 | 0.2 |
| U09350 | VIP | 2.0 | 2.4 | 0.2 | 0.1 | 0.3 | 0.2 | 0.2 | 0.2 |
| U62026 | CENPF | 3.3 | 9.9 | 0.7 | 0.7 | 1.4 | 2.2 | 0.6 | 0.6 |
| M16199 | IL8 | 3.7 | 3.0 | 22.4 | 43.4 | 26.8 | 172.7 | 18.5 | 39.0 |
| X02009 | LTF | 0.7 | 0.5 | 2.7 | 2.3 | 3.6 | 5.2 | 3.2 | 4.1 |
| AJ004940 | HSPA8 | 0.9 | 1.1 | 0.5 | 0.4 | 0.6 | 0.5 | 2.7 | 3.2 |
| AF411083 | SFTPA1 | 1.7 | 2.5 | 0.4 | 0.7 | 0.9 | 0.9 | 0.4 | 0.3 |
| X16881 | CDC2 | 1.9 | 2.2 | 0.4 | 0.3 | 0.7 | 0.6 | 0.4 | 0.4 |
| U12438 | RFC2 | 1.9 | 2.0 | 0.8 | 0.6 | 1.0 | 0.9 | 0.3 | 0.2 |
| Y14971 | CXC chemokine K60 | 3.7 | 7.9 | 17.3 | 60.3 | 19.7 | 206.4 | 11.3 | 38.2 |
The gene expression levels of 20 genes at four different time points in microarray analysis were confirmed by qRT-PCR. The expression levels were presented by fold changes values in microarray analysis, while, for qRT-PCR, the values were calculated by 2-ΔΔCT method [31], which would be comparable to fold changes in the microarray. All values are mean values determined by the calculation from three replicating assays.