Table 1.
Structural and evolutionary characteristics of the 5 main groups of IS elements characterized in the genome of S. glossinidius.
| ISSgl1 | ISSgl2 | ISSgl3 | ISSgl4 | ISSgl5 | ||
|---|---|---|---|---|---|---|
| IS family | IS5 | IS110 | IS256 | IS110 | ISNCY | |
| Structural features | ||||||
| Consensus sequence length (bp) | 1052 | 1175 | 1247 | 1210 | 939 | |
| GC content (%) | 49.8 | 49.7 | 51 | 48.7 | 52.7 | |
| Number of open reading frames | 1 | 1 | 1 | 1 | 1 | |
| Inverted repeats (bp) | 17 | 11 | 34 | 10 | n.d. (c) | |
| Direct repeats derived from transposition (bp) | 9 | n.d. (d) | 9 | n.d. (d) | n.d. (d) | |
| Complete copies (a) | 47 | 9 | 7 | 7 | 4 | |
| Partial copies (b) | 16 | 9 | 12 | 2 | 9 | |
| IS with functional transposase genes | 10 | 4 | 0 | 0 | 0 | |
| Pseudogenes by IS insertion | 10 | 2 | 2 | 3 | 0 | |
| p-distance (number of nucleotide differences/total length) | ||||||
| Minimum | 0.0 | 0.0024 | 0.0062 | 0.0048 | 0.1097 | |
| Maximum | 0.066 | 0.0268 | 0.0756 | 0.0240 | 0.3935 | |
| Mean | 0.02 | 0.0162 | 0.0287 | 0.0131 | 0.2388 | |
| Total length (nt) (e) | 52130 | 15328 | 16915 | 9375 | 11411 | |
a Complete copies are those that present inverted repeats at both ends of the transposase gene.
b Partial copies correspond to fragments of the putative IS element.
c No inverted repeats were detected in ISSgl5
d No direct repeats were detected in ISSgl2, ISSgl4, and ISSgl5 (n.d.).
e Sum of the length of all corresponding complete and partial IS elements.