Table 1.
Overview of the results comparing use of oligo(dT) and multi-targeted primers (MTPs) for reverse transcription.
| Saccharomyces cerevisiae | Neurospora crassa | |||||
|---|---|---|---|---|---|---|
| Feature | Nitrogen depletion | Protoperithecial developmenr | ||||
| Oligo (dT) | MTP | Common | Oligo (dT) | MTP | Common | |
| Number of well measured genes1 on the array | 4620 | 6042 | 4573 | 469 | 416 | |
| Number of genes significantly differentially expressed (P ≤ 0.05) | 2155 | 3577 | 1839 | 172 | 406 | 2021 |
| Number of up-regulated genes (P ≤ 0.05) | 1080 | 1855 | 925 | 66 | 232 | 31 |
| Number of down-regulated genes (P ≤ 0.05) | 1075 | 1722 | 912 | 106 | 174 | 61 |
| Highest gene expression ratio | 49.15 | 32.6 | 24.26 | 10.74 | ||
| Average gene expression ratio | 2.05 | 1.77 | 1.96 | 1.43 | ||
| GEL502 | 1.51 | 1.27 | 1.91 | 2.08 | ||
| Number of genes with gene expression ratio greater or equal to GEL50 (P ≤ 0.05) | 1237 | 2617 | 1166 | 102 | 141 | 52 |
1 well measured genes recorded after data normalization (foreground fluorescence signals were at least three standard deviations of the distribution of intensities of the background pixels for that gene).
2 GEL50 - gene expression level at which there is a 50% empirical probability of a significant call.