Table 1.
Quantity of starting RNA: | 250 ng | 100 ng | 50 ng | 10 ng |
---|---|---|---|---|
Total decoded |
18,801,235 |
17,835,076 |
17,926,750 |
19,274,434 |
Removed by BASH |
200,459 |
408,721 |
284,088 |
50,449 |
Removed in summarization |
651,323 |
614,495 |
603,619 |
582,345 |
Remaining |
17,949,453 |
16,811,860 |
17,039,043 |
18,641,640 |
In analysis-group bead-types | 7,963,638 | 7,475,940 | 7,563,440 | 8,248,259 |
Summing across all array-sections in the four experiments, we list the total numbers of beads (as decoded by Illumina), the numbers we remove as being in spatial artefacts using BASH [19], those removed as being outliers in the summarization, the remainders, and the numbers remaining that lie in the analysis-group of bead-types.