Table 4.
Control bead-type summary
| Quantity of starting RNA: | 250 ng | 100 ng | 50 ng | 10 ng |
|---|---|---|---|---|
| Negative controls detected in UHRR |
37 |
32 |
20 |
0 |
| Negative controls detected in Brain |
35 |
40 |
29 |
24 |
| Negative controls detected in all array-sections |
10 |
6 |
2 |
0 |
| Negative controls detected in at least one array-section |
88 |
89 |
98 |
99 |
| Negative controls: median log-intensity UHRR |
5.78 |
5.79 |
5.73 |
5.58 |
| Negative controls: median log-intensity Brain |
5.80 |
5.82 |
5.72 |
5.58 |
| Housekeeping controls: median log-intensity UHRR |
13.46 |
13.14 |
13.09 |
11.32 |
| Housekeeping controls: median log-intensity Brain | 12.47 | 12.52 | 12.03 | 10.64 |
Giving a) the numbers of the 759 negative-control bead-types that show expression in one or more array-sections, and b) median log-intensities for negative-control and housekeeping bead-types in 100% UHRR and 100% Brain array-sections.