Figure 6.

Molecular interactions between zinc and proteins encoded by genes changed under zinc depletion. MTF1 was added to the input list although its mRNA expression was not changed in the present study. (A) A Direct Interaction Network based on curated interactions contained within the PathwayArchitect database (Additional file 2, Figure S1). Red ovals represent proteins and the blue circle symbolises Zn(II). Dark blue squares denote 'binding', and light blue squares 'expression'; green squares stand for 'regulation', green diamonds for 'metabolism', and green circles for 'promoter binding'. Arrow heads indicate directionality of the interaction where annotated. (B) Heatmap of expression levels of transcripts that interact directly with HNF4A during 14 days of zinc depletion.