Table 1.

Primers used in qPCR validation and the result from qPCR and microarray measurements.

		Primers		Depletionb
Gene ID (symbol)a	Time point	Forward (5'-3')	Reverse (5'-3')	Array	qPCR	Array	qPCR
Dr.134300 (ppara)	24 h	GAACGCAACTGCAAGATCCAG	CCAAAACGAATAGCGTTGTGG	1.21 ± 0.5	0.99 ± 0.2	1.98 ± 0.2	1.52 ± 0.1
Dr.15390 (foxl1)	24 h	TGGTGTTTGGCATCCTCGAC	CTTGAAAGCGGTGTGTGAGTG	2.42 ± 1.0	0.97 ± 0.2	1.47 ± 0.4	0.84 ± 0.1
Dr.77183 (hmgcs1)	24 h	GTCACTCAAGATGGCACACCTG	CTCTGAGCTTCATTGTCTCAGC	1.36 ± 0.1	1.31 ± 0.4	1.57 ± 0.8	1.46 ± 0.8
Dr.76570 (apob)	24 h	TCAGCAATGCTATTCGCATGAG	CACTTATCCCATCACTCCGAGC	1.36 ± 0.5	1.04 ± 0.8	2.25 ± 0.6	2.06 ± 1.0
Dr.80336 (cyp11a1)	7d	GCAGCCCTGAAAGAAACTCTCA	TCCCTGCTGGAATGTGGTAATT	0.72 ± 0.4	2.72 ± 1.0	0.53 ± 0.2	0.41 ± 0.1
Dr.429 (dlx5)	7d	ACGCGAGCTCTTCGTGGTATT	CAGTACAACGTTCCTGATCCGA	0.38 ± 0.1	0.56 ± 0.2	0.56 ± 0.3	0.96 ± 0.5
Dr.2 (dlx2)	7d	GCCTCACGCAAACACAGGTT	AGACTCACCGGAGGCCACAT	1.08 ± 0.6	1.20 ± 0.5	1.87 ± 0.6	2.28 ± 1.2
Dr.78697 (rfx2)	14d	CCGGAGATCATCAGCACTAAGG	GGTCGCTGAGCATCTGATTGA	1.26 ± 0.8	0.75 ± 0.1	2.07 ± 0.3	2.99 ± 1.5
Dr.121290 (mt)	8 h	AATGGACCCCTGCGAAT	GGTAGCACCACAGTTGCAA	0.32 ± 0.3	0.30 ± 0.1	1.91 ± 1.5	1.87 ± 1.2
	24 h			0.21 ± 0.2	0.81 ± 0.4	3.67 ± 0.6	2.72 ± 0.9
	4d			0.53 ± 0.2	0.44 ± 0.2	4.90 ± 1.7	4.91 ± 2.9
	7d			0.15 ± 0.1	0.38 ± 0.1	7.48 ± 1.6	5.65 ± 1.4
	14d			0.91 ± 0.7	0.28 ± 0.1	13.82 ± 3.3	12.52 ± 4.4
Dr.12303 (znt1)	8 h	AGTGCCCGAGCAGATCGA	GCTAGAACTCCATCCAGGCTCTT	0.74 ± 0.2	0.82 ± 0.1	1.11 ± 0.0	1.30 ± 0.4
	24 h			0.60 ± 0.4	0.94 ± 0.6	1.42 ± 0.4	1.11 ± 0.4
	4d			0.89 ± 0.0	0.78 ± 0.2	0.88 ± 0.2	1.22 ± 0.2
	7d			0.98 ± 0.0	0.55 ± 0.2	2.01 ± 0.6	1.57 ± 0.4
	14d			0.92 ± 0.3	0.96 ± 0.4	1.35 ± 0.6	2.71 ± 0.9
18s		CGGAGGTTCGAAGACGATCA	CGGGTCGGCATCGTTTAC

^aGene IDs are provided as UnigeneID and ZNC gene symbols

^bExpression levels are expressed as mean ± SD of fish treated with zinc depletion or supplementation compared to control as analysed by the two methods for the durations indicated

^cSupplementation was conducted as a parallel experiment [30].