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. 2010 Oct 11;11:558. doi: 10.1186/1471-2164-11-558

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Copyright ©2010 Ellis and Carney; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Candidate genes are expressed in fat tissue. Antisense (A-D,I,K,M-Q) or sense (E-H,J,L,R-V) RNA probes were designed to cDNA clones for CG4825 (A,E,M,R), CG8449 (B,F,I,J,N,S), bgm (C,G,O,T), Prx2540-2 (D,H,K,L,P,U), and CG13360 (Q,V). In situ hybridization to whole-mount tissue showed candidate gene expression in male CS fat body tissue (arrows) present on head (A-H) and abdominal (M-V) cuticle. Purple reactivity indicates presence of transcripts. Brains (I-L) showed light pink background staining but lacked detectable expression of either CG8449 (I,J) or Prx2540-2 (K,L).