Table 2.

Candidate genes down regulated 2 hrs after mating

Gene identifier	Gene name	Avg. fold change	GO Molecular function	GO Biological process
CG1897	Drop (Dr)	-1.5	DNA binding	Central nervous system development
CG2505	α-Esterase-2 (α-Est2)	-1.3	Carboxylesterase activity	Unknown
CG3200	Rhythmically expressed gene 2 (Reg-2)	-1.27	Phosphoglycolate phosphatase activity	Metabolic process
CG3926	Serine pyruvate aminotrans-ferase (Spat)	-1.34	Serine-pyruvate transamine activity	Glyoxylate catabolic process
CG4105	Cytochrome P450-4e3 (Cyp4e3)	-1.3	Electron carrier activity	Unknown
CG5840		-1.34	Pyrroline-5-carboxylate reductase activity	Proline biosynthetic process
CG6806	Larval serum protein 2 (Lsp2)	-1.28	Nutrient reservoir activity	Transport
CG7224		-1.16	Unknown	Unknown
CG7390	senescence marker protein-30 (smp-30)	-1.36	Unknown	Unknown
CG8112		-1.42	Sterol O-acyltransferase activity	Unknown
CG8846	Thor	-1.26	Eukaryotic initation factor 4E binding	Immune response
CG9416		-1.25	Sequence-specific DNA binding	Regulation of transcription
CG9733		-1.6	Trypsin activity	Proteolysis
CG11909	target of brain insulin (tobi)	-1.42	α-glucosidase activity	Carbohydrate metabolic process
CG11919		-1.36	ATP binding	Peroxisome organization and biogenesis
CG16898		-1.68	Unknown	Unknown
CG18003		-1.36	Glycolate oxidase activity	Metabolic process
CG30489	Cyp12d1-p	-1.3	Electron carrier activity	Unknown
CG31075		-1.26	Aldehyde dehydrogenase (NAD) activity	Pyruvate metabolic process
CG31628	adenosine 3 (ade3)	-1.28	Phosphoribo-sylamine-glycine ligase activity	Purine base biosynthetic process
CG31689		-1.25	ATPase activity	Unknown
CG33462		-4.08	Trypsin activity	Proteolysis

Average fold changes, molecular functions and biological processes are shown for 22 genes that are significantly (p < 0.001) down regulated in male heads 2 hrs after mating.