Table 3.
Confirmation of microarray results by qPCR
| Gene identifier | Gene symbol | Microarray Fold change | qPCR Relative fold change ± SEM | Avg. relative expression level in control male heads ± SEM | Avg. relative expression level in mated male heads ± SEM |
|---|---|---|---|---|---|
| CG5618 | 1.14 | 2.02 ± 0.49* | 0.36 ± 0.09 | 0.74 ± 0.18 | |
| CG6188 | 1.64 | 1.94 ± 0.26* | 2.25 ± 0.42 | 4.38 ± 0.58 | |
| CG8449 | 1.28 | 1.35 ± 0.15* | 1.24 ± 0.26 | 1.68 ± 0.18 | |
| CG16772 | 1.5 | 4.07 ± 1.55 | 6.86 ± 1.72 | 27.94 ± 10.61 | |
| CG30026 | 1.42 | 2.23 ± 0.35* | 4.36 ± 0.84 | 9.74 ± 1.53 | |
| CG4501 | bgm | 1.38 | 4.47 ± 1.11* | 1.42 ± 0.31 | 6.37 ± 1.57 |
| CG6342 | Irp-1B | 1.26 | 1.42 ± 0.12 | 1.09 ± 0.18 | 1.55 ± 0.13 |
| CG11765 | Prx2540-2 | 1.2 | 1.23 ± 0.12* | 0.47 ± 0.08 | 0.58 ± 0.06 |
| CG2505 | αEst2 | -1.3 | -1.26 ± 0.15 | 2.84 ± 0.59 | 2.25 ± 0.42 |
| CG7390 | smp-30 | -1.36 | -1.69 ± 0.16* | 1.28 ± 0.45 | 0.77 ± 0.2 |
* Indicates a significant (p < 0.05) difference between the average relative expression level in control male heads and mated male heads. SEM = Standard error of the mean.