Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Semi-quantitative Reverse Transcription-PCR (RT-PCR) of candidate genes. Normalized cDNA obtained from reverse transcription of mRNA was used as template for PCR amplifications. Aliquots were taken from PCR reactions at 20, 25 and 30 cycles and analyzed by agarose gel electrophoresis.
