Table 4.
Forward and reverse primer sequences used in semi quantitative RT-PCR analyses.
| Candidate gene | GenBank acc. no | 5'-3' forward primer | 5'-3' reverse primer |
|---|---|---|---|
| RECOGNITION | |||
| Galectin | HM756110 | CTCCGGCGGGAGGGAATCCA | AGTGGAAGCTGGGGTTCCGAGG |
| Carcinolectin | HM756111 | CGGATACAGTGGCACGGCAG | TGATACCAACGAGCACCAGCAC |
| Aggrecan | HM756112 | TGCAAGCGGATACCCGGTAAA | ATCAACGCAGAGTGGCCGAG |
| C-lectin | HM756113 | AGGCTTGGGATAGGCACATGGA | ACGATTCACCCGAACAGAGTTGG |
| LBP/BPI | HM756114 | GCTTCACTGATACTGCTTGCCC | CCACGGTGGAGCAGCATGGA |
| SRCR | HM756115 | TGATTCGATACCAAGGACCCAAAGGT | TGTCAACTCCGGCTATTCCAGGT |
| PGRP | HM756116 | TCACACGGAAGGAGGAGCGT | AGGGCTGCCTTGGATGGTGT |
| Thrombospondin | HM756117 | TGCTGCGACCCATTTCTGTGA | GTGAGGAGTTCCACTGGTGAGGG |
| Rhamnose-binding lectin | HM756118 | ACAATGGGTTGATTTGTTTGCCGA | CCGGGGGCCTGAAAGTTGGT |
| SIGNALING | |||
| Lipoprotein receptor | HM756119 | CAGAGCCATCCACTTTGGCGG | AGGTCTACACCTTCCAGCAGCA |
| Integrin | HM756120 | ACGGCCGGGGAGAAGTTGAA | CGCAGTCACACGTTCCACAGAC |
| Serpin | HM756121 | AGGGTTGTGCGTGAAGTGGA | TCTCAAAGCGAGGCTGCCAGA |
| α-macroglobulin receptor | HM756122 | CATTACGGCCGGGGCAAAGG | TGCTGGCTCTCTCAGCTCGG |
| Tenascin | HM756123 | CATTACGGCCGGGGGTTGTA | AGTCGGAACAGTCCTTTGGGT |
| EGF | HM756124 | GGGACACATTGCGAAACGGC | TTGCCCCGTAAATCCAGGCA |
| GTPase | HM756125 | ATTACGGCCGGGGGACACAC | TTCGGCATCCTGGCACTTCG |
| TNF factor | HM756126 | GGGATTAGGCAACACCCAAGCC | CCGCCACAGTACAGCCAACC |
| VEGF receptor | HM756127 | AGCTGCATGGAGACTTGAACCAGA | AGGTGGGGTGGTACTTGCTCC |
| Fas ligand | HM756128 | CGATTCGCTAGGACCGGGGA | AGTCATTGGCGGTACTCCACACA |
| Toll | HM756129 | AGGAGGACTCGGATGACACAGC | ACTCCGGAACTTGGAGAGCACG |
| MyD88 | HM756130 | CTGCCACACCCAACAACGCA | TCGAGACTGAGGTTCTCGCACA |
| TRAF | HM756132 | CCCAACGACAGCCTTCTTTGACG | ATTACGGCCGGGGGCTTGTT |
| IRAK | HM756133 | GAGTGGCCATTACGGCCGGG | GCTTGCATCGATCTGGCGGGT |
| TRAF 6 | HM756134 | CACCTATTTCCGCTTCCCGCC | TGGAGGGTGGTGGTGCTCTT |
| TRANSCRIPTION | |||
| NF-κB | HM756136 | CCAAATGATGCACCTGCTCTTTTCAGT | CATTACGGCCGGGGAAGGGA |
| Tal | HM756137 | GTTGACGCCATCGCTCTCGG | GCCATTACGGCCGGGGTTTA |
| Jun | HM756138 | CGCCAACACCGACACAGTTCA | AACCCCCGGGGAGTGTTGTT |
| AP-1 | HM756139 | TGCAGCTACACGGTTTCTGGC | TCGGCAACAACACTCCCC |
| I-κB | HM756140 | TGAGGCAGCACTGAACGGAC | CGCAGAGTGTGCCAACAGCA |
| STAT long form | HM756141 | ACGGCCGGGGTAAAGCTGAA | ACAAATCCAGCCACATGCCCA |
| STAT (SH2 motif) | HM756142 | AGCGTCAAACACGACAGACGA | AGACCACGCCCTGTTTCAGC |
| EFFECTOR | |||
| Cytolysin | HM756143 | CGGTTGCTGTGTAGCCGCAT | GGCGTCCAGAGACCGGAGTT |
| Glutathione peroxidase | HM756144 | TTAACGGCGTCGTCGCTTGG | TGGCTTCTCTCTGAGGAACAACTG |
| TIMP | HM756145 | TGTCCCATGGGTCTGGAACGG | TCAGCCTGTTCCTCTTGGCATT |
| Thiolester- macroglobulin | HM756146 | CTGGCTCTCTCAGCTCGGCA | GGGCACTCTCCGGTCTTGGT |
| Metallothionein 1B | HM756147 | TCGGCACTGTCCACACAAAACC | CAACCGGAAGCGGATGTGGC |
| Big Defensin | HM756148 | CCGGGGGCGATTGCCTTTC | ACCAAGGCCCAAAATGCAGC |
| Defensin | HM756149 | AACGCAGAGTGGGCCATACG | TCACTGGTGCGAACCGTTTGT |
| Ferritin | HM756151 | TCAACGCAGAGTGGGGCCAT | GCGGTTCAGAAGTTGTTGTCACG |
| Catalase | HM756152 | CATGTTAGCAGGCACTCCAGACC | TACGGCCGGGGGAAAAAGGT |
Based on sequences retrieved from the DeepSeaVent database, primers were designed to confirm, in RT-PCR experiments, the physical counterpart of B. azoricus genes putatively associated with immunity and inflammatory reactions.