Table 3.
Primers and PCR product size of the genes used for semi-quantitative RT-PCR analysis
| S.No | Gene Name | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|---|
| 1. | Carboxyl transferase of ACCase β subunit (CT-β) | GAATGAGTTACTTCAGCTTCAC | AATTCACCCTTCTTTCTTGTTGA | 307 |
| 2. | Biotin carboxyl carrier protein I of ACCase (BCCPI) | GTCGGCTAATCTTAAAGCTATTC | TGTTTATAGCTTCACTAGTGTAC | 258 |
| 3. | Biotin carboxyl carrier protein II of ACCase (BCCPII) | GATGCTCTCATTGCAATTCTC | TAATGATAAATAACAATGAAGAAGG | 278 |
| 4. | Malonyl-CoA:ACP transacylase (MCAT) | ACTTCTCCTGTTCAATGGGAA | TCTCTAGTACATGACGCTAATC | 314 |
| 5. | 3-keto acyl ACP Reductase (KAR) | GTTATCTCTCCCGAAAGTGTA | CACAGTATCTGTCACCTTTTC | 306 |
| 6. | Beta-keto acyl ACP synthase I (KASI) | GCATCTGGCTTGTCTCCAT | GCAGAAGAACACAATTTTGATAC | 402 |
| 7. | Beta-keto acyl ACP synthase II (KASII) | TGTTCCCAATTTGAAGAAGCAG | AGGAACACCAAATCCAATCTTAT | 307 |
| 8. | Acyl carrier Protein (ACP) | AAGATCAGTCTAGGAAATCCTTC | GCATAAATTAGGAAATTTTAGAGTGT | 180 |
| 9. | Acyl ACP thioesterase (FATA) | AATAATGTAGATTTCTTTATTTGTGT | AGTAAACGTAAAACAATACAGTTGAT | 285 |
| 10. | ω-3-fatty acid Desaturase (ω-3-FAD) | AAAGCTGCAAAATTTTTTATCTGCA | CCCTCTCAAATCCAATCCAA | 215 |
| 11. | Acyl -CoA binding protein (ACBP) | TGAACATTTCTATGCCGCTTG | GAGAAATAAGGGTCACCATTATC | 310 |
| 12. | Long chain acyl-CoA synthetase (LCACS) | ATCCATAGCATTTGGCTTTCAAA | CGTGTAGAAGATGAATTGTATAAC | 326 |
| 13. | Acyl-CoA thioesterase (ACT) | ACTCATCTTAGCTTTGTTATGTTC | CCCATTTCAATCACCGTTTC | 173 |
| 14. | Glycerol-3-phosphate acyl transferase (GPAT) | GGGTAACGTGTTGGGATTTG | CACAGAATCCAAATAATTCTACATTT | 200 |
| 15. | Lysophosphatidic acid acyl transferase (LPAT) | TGGTGTATGTTTGTGCTTGG | CTGTACAAAATTGAATCAAGCTTTTT | 238 |
| 16. | Diacyl glycerol kinase (DGK) | CGGCTATTCGGTTGGAAATAA | GATTTTTGATACAACAAATTACCAGT | 296 |
| 17. | Triacyl glycerol lipase(TAGL) | CTGGAGACAAGACGAGAATAA | CTTCTATCATAATCAATTATTGTTCG | 310 |
| 18. | Actin | CAAGTCATCACCATTGGAGCA | GCCTCTTAATTTCGGCTTTAACA | 590 |