Table 1.
Changes in gene expression in BMDCs in response to treatment with [BF/S+L/Ep]
| Expression change relative to DMSO alone | Up-regulated genes | Down-regulated genes | Total number of regulated genes | Percentage of all genes (%) | |
|---|---|---|---|---|---|
| At 4 h: | |||||
| Total genes | Significant a | 513 | 472 | 985 | 2.90 |
| ≥ 1.5 | 104 | 94 | 198 | 0.58 | |
| ≥ 2 b | 19 | 10 | 29 | 0.09 | |
| ≥ 3 | 6 | 5 | 11 | 0.03 | |
| ≥ 4 | 3 | 2 | 5 | 0.01 | |
| Immune-related | Significant a | 89 | 83 | 172 | 0.51 |
| ≥ 1.5 | 16 | 14 | 30 | 0.09 | |
| ≥ 2 c | 4 | 0 | 4 | 0.01 | |
| ≥ 3 | 2 | 0 | 2 | 0.01 | |
| ≥ 4 | 0 | 0 | 0 | 0 | |
|
Non-immune- related |
≥ 2 b-c | 15 | 10 | 25 | 0.07 |
| At 12 h: | |||||
| Total Genes | Significant a | 605 | 736 | 1341 | 3.94 |
| ≥ 1.5 | 128 | 132 | 260 | 0.76 | |
| ≥ 2 d | 21 | 18 | 39 | 0.11 | |
| ≥ 3 | 6 | 5 | 11 | 0.03 | |
| ≥ 4 | 3 | 3 | 6 | 0.02 | |
| Immune-related | Significant a | 144 | 120 | 264 | 0.78 |
| ≥ 1.5 | 27 | 19 | 46 | 0.14 | |
| ≥ 2 e | 2 | 0 | 2 | 0.01 | |
| ≥ 3 | 1 | 0 | 1 | 0 | |
| ≥ 4 | 1 | 0 | 1 | 0 | |
|
Non-immune- related |
≥ 2 d-e | 19 | 18 | 37 | 0.11 |
a Differentially expressed genes that are significant in [BF/S+L/Ep] vs. [DMSO] (control). Values of p ≤ 0.05 are considered to be significant. All relative differences greater than 1.5 are significant.
b Up-regulated genes with at least a 2-fold change in the level of RNA transcript expression after 4 h treatment
c Up-regulated immune-related genes with at least a 2-fold change after 4 h treatment
d Down-regulated genes with at least a 2-fold change in the level of RNA transcript expression after 4 h treatment
e Down-regulated immune-related genes with at least a 2-fold change after 12 h treatment