Table 5.
Candidate genes for the improvement of malting quality.
| Probe Set Name | E value | Putative function | ME | GP | S/T | DP | AA | BG | Map loc. |
|---|---|---|---|---|---|---|---|---|---|
| "Out of steep" | |||||||||
| Contig10584_at | _ | No hit | **(+) | ||||||
| Contig12460_at | 7.00E-22 | Hypothetical protein P0657H12.23 (Oryza sativa) | **(-) | *(+) | |||||
| Contig13150_at | _ | No hit | *(+) | ||||||
| Contig14046_at | 1.00E-21 | Hypothetical protein OJ1756_H07 (Oryza sativa) | *(-) | *(-) | |||||
| Contig17218_at | _ | No hit | **(+) | *(+) | |||||
| Contig17647_at | 2.00E-29 | Os08g0100400 protein | *(-) | *(-) | |||||
| Contig21675_at | _ | No hit | ***(+) | **(+) | *(-) | ||||
| Contig22323_at | 6.00E-38 | 26S protease regulatory subunit 6B | **(+) | *(+) | |||||
| Contig23156_at | _ | No hit | *(-) | *(-) | **(+) | ||||
| Contig25703_at | 2.00E-32 | F-box domain containing protein | **(+) | *(-) | |||||
| Contig2885_at | 4.00E-60 | Eukaryotic translation initiation factor 5 (Zea mays) | *(+) | **(-) | |||||
| Contig3129_at | 1.00E-124 | Mitochondrial prohibitin complex protein 2 (Oryza sativa) | *(-) | **(-) | *(-) | ||||
| Contig3173_at | 2.00E-23 | Low temperature and salt responsive protein (Pennisetum americanum) |
*(+) | 2H (51.75) | |||||
| Contig3191_at | 1.00E-121 | Mitochondrial ATP synthase (Triticum aestivum) | *(+) | ||||||
| Contig3339_at | 1.00E-115 | Protein CP5 | *(+) | *(-) | |||||
| Contig3668_at | 1.00E-115 | Ice recrystallization inhibition protein 1 precursor (Triticum aestivum) |
*(-) | ||||||
| Contig3861_at | _ | No hit | *(-) | *(-) | *(-) | ***(-) | |||
| Contig3863_s_at | _ | No hit | *(-) | *(-) | **(-) | ||||
| Contig398_s_at | 6.00E-91 | 6,7-dimethyl-8-ribityllumazine synthase, chloroplast precursor | *(-) | ||||||
| Contig4837_at | 1.00E-52 | Small nuclear ribonucleoprotein Sm D2 | *(+) | **(+) | |||||
| Contig4839_at | 5.00E-52 | Small nuclear ribonucleoprotein Sm D2 | *(-) | ||||||
| Contig6845_at | _ | No hit | *(-) | *(-) | |||||
| Contig9672_at | 7.00E-52 | Unknown | *(+) | *(+) | **(+) | 3H (162.15) | |||
| Contig9733_at | 9.00E-98 | RING-H2 finger protein | *(-) | ||||||
| EBem10_SQ004_D16_at | _ | No hit | *(+) | *(-) | |||||
| EBpi01_SQ001_C06_at | _ | No hit | ***(-) | *(-) | |||||
| HB20B24r_s_at | 2.00E-85 | Hordoindoline b (Hordeum vulgare) | *(+) | ||||||
| HS04A09u_s_at | _ | No hit | ***(+) | *(+) | 7H (140.21) | ||||
| HT12D12u_s_at | 5.00E-48 | Glutaredoxin (Triticum aestivum) | *(-) | ||||||
| HV_CEa0001D21r2_at | _ | No hit | **(+) | *(-) | 2HS | ||||
| HV_CEa0011J09r2_at | 2.00E-18 | Putative B12D protein (Oryza sativa) | **(-) | **(+) | 1H (128.14) | ||||
| HVSMEa0001B10r2_x_at | _ | No hit | *(-) | ||||||
| HVSMEl0003B06r2_at | _ | No hit | **(+) | *(+) | |||||
| HVSMEl0007F21r2_s_at | _ | No hit | **(+) | ||||||
| HVSMEl0023H09r2_at | _ | No hit | *(-) | ||||||
| rbags14a14_at | 2.00E-36 | Putative prolyl endopeptidase (Oryza sativa) | *(-) | ||||||
| rbags14a14_s_at | 2.00E-36 | Putative prolyl endopeptidase (Oryza sativa) | **(-) | 6H (45.44)\ | |||||
| "3d of germination" | |||||||||
| Contig12150_at | _ | No hit | *(-) | *(-) | 7H (140.21) | ||||
| Contig12460_at | 7.00E-22 | Hypothetical protein P0657H12.23 (Oryza sativa) | **(-) | *(-) | *(+) | ||||
| Contig13847_s_at | 3.00E-85 | Putative subtilisin-like serine protease (Oryza sativa) | **(+) | *(-) | |||||
| Contig14046_at | 1.00E-21 | Hypothetical protein OJ1756_H07 (Oryza sativa) | **(-) | *(-) | |||||
| Contig14709_at | 3.00E-37 | Os01g0579800 protein | **(-) | *(-) | *(-) | 1H (138.31) | |||
| Contig159_at | 3.00E-53 | Histone H4 | *(+) | *(+) | *(+) | ***(+) | |||
| Contig16647_at | 6.00E-42 | Os12g0566100 protein | **(-) | *(-) | |||||
| Contig17218_at | _ | No hit | **(+) | *(+) | |||||
| Contig173_at | 6.00E-54 | Histone H4 | *(-) | ||||||
| Contig17647_at | 2.00E-29 | Os08g0100400 protein | **(-) | *(-) | |||||
| Contig22323_at | 6.00E-38 | 26S protease regulatory subunit 6B | **(+) | *(+) | |||||
| Contig2885_at | 4.00E-60 | Eukaryotic translation initiation factor 5 (Zea mays) | *(+) | *(+) | **(-) | ||||
| Contig3129_at | 1.00E-124 | Mitochondrial prohibitin complex protein 2 (Oryza sativa) | **(-) | *(-) | |||||
| Contig3668_at | 1.00E-115 | Ice recrystallization inhibition protein 1 precursor (Triticum aestivum) |
*(-) | ||||||
| Contig3863_s_at | _ | No hit | *(-) | *(-) | **(-) | ||||
| Contig4384_at | 1.00E-92 | DAG protein, chloroplast precursor | *(-) | 5H (103.92) | |||||
| Contig500_at | 2.00E-53 | Histone H4 | *(-) | *(-) | **(-) | ||||
| Contig5185_at | 1.00E-145 | RNase S-like protein (Hordeum vulgare) | *(-) | 1H (135.56) | |||||
| Contig6845_at | _ | No hit | **(-) | *(-) | |||||
| Contig7285_at | 2.00E-96 | Hypothetical protein P0576F08.31(Oryza sativa) | *(+) | 6H (43.15) | |||||
| Contig7399_s_at | 1.00E-115 | Ubiquitin carboxyl-terminal hydrolase isozyme L3 | **(+) | **(+) | *(-) | 6H (60.23) | |||
| HB27E22r_at | _ | No hit | *(+) | ||||||
| HS04A09u_s_at | _ | No hit | ***(+) | *(+) | 7H (140.21) | ||||
| HVSMEl0003B06r2_at | _ | No hit | **(+) | *(+) | |||||
| HVSMEl0007F21r2_s_at | _ | No hit | *(+) | ||||||
*, **, ***: Significant correlation at p < .05, p < .01, and p < .001, respectively. +: positive correlation, -: negative correlation. Gene annotations were exported from HarvEST:Barley version 1.68. ME: malt extract, GP: grain protein content, S/T: ratio of wort soluble protein to total malt protein, DP: diastatic power, AA: alpha-amylase activity, BG: malt beta-glucan content. Bold type indicates common genes to both time points. The genetic map locations (map loc.) were based on the barley SNP map [24].