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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Neuropeptides. 2011 Apr 19;45(3):213–218. doi: 10.1016/j.npep.2011.03.003

Figure 2.

Figure 2

Heparin affinity chromatography of the DNSPs and GDNF. 10 μM samples of the synthetic DNSPs and GDNF (in 10 mM sodium citrate, pH 5.6) were applied to a HiTrap™ Heparin HP affinity column and elutant was monitored at 215 nm. After sample loading and wash, a high salt (10 mM sodium citrate + 2 M NaCl) linear gradient was applied (dashed line) to elute heparin-binding samples.