Figure 6. Comparison of virus titre during infection in the presence or absence of cytokine-containing supernatants.

Cytokine supernatants collected from primary human monocytes infected at an MOI of 0.1 with either TCRV or JUNV or mock infected at 4 days post-infection were subjected to ultracentifugation to remove virus particles. Macrophage cultures were then pretreated with the clarified supernatant of TCRV, JUNV or mock infected monocytes for 2 h prior to the addition of known amounts of (A) JUNV or (B) TCRV for infection. Samples were collected after 4 days and plaque assays performed to determine virus titres.