(A) Comparison of Tm-FliGMC(ΔPEV) and wild-type
Tm-FliGMC (PDB code 1lkv). A
FliGM-FliGC unit of wild-type
Tm-FliGMC, which is composed of FliGM of one
subunit and FliGC of the neighboring subunit related by
2-fold crystallographic symmetry, is superimposed onto
Tm-FliGMC(ΔPEV) using Cα atoms of V117-L165 and
G196-F236 for least-square fitting. FliGM with helix E and
FliGC of Tm-FliGMC(ΔPEV) are colored cyan
and blue, respectively. FliGM with helix E and
FliGC of wild-type Tm-FliGMC are yellow and
orange, respectively. (B) Comparison of Tm-FliGMC(ΔPEV)
with Aa-FliG (PDB code 3hjl). A FliGM-FliGC unit
of Aa-FliG, which is composed of FliGM of one molecule and
FliGC of the neighboring molecule related by 2-fold
crystallographic symmetry, is superimposed onto
Tm-FliGMC(ΔPEV) using Cα atoms of the same region
used in (A). Tm-FliGMC(ΔPEV) is shown in the same color
as in (A), and FliGM and FliGC of
Aa-FliGMC are shown in green and red, respectively. (C)
Comparison of the orientation of helix E. The
FliGM-FliGCN units of wild-type
Tm-FliGMC and wild-type Aa-FliGMC are
superimposed on Tm-FliGMC(ΔPEV). The models are shown in
the same colors used in (A) and (B).