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. 2011 May 10;6(5):e18553. doi: 10.1371/journal.pone.0018553

Figure 5. Production of IFN-γ by the sub-population of resting human peripheral blood CD4+ T cells after activation with IL-12 plus IL-18.

Figure 5

(A) Naïve (RA+) and memory (RO+) cells isolated from human peripheral blood CD4+ T cells were stimulated with IL-12 and IL-18 for 48 hours in the presence or absence of SB203580. Supernatants were analyzed for IFN-γ production. (B) Responsiveness of IL-18Rα+ T cells to IL-12 plus IL-18. Freshly isolated IL-18Rα+ T cells from naïve (RA+18+) and memory (RO+18+) CD4+ T cells were treated with IL-12 plus IL-18 for 48 hours in the presence or absence of SB203580. Supernatants were analyzed for IFN-γ production. (C) IL-18Rα+ (upper panel, 18+) and IL-18Rα− (lower panel, 18−) T cells from naïve (CD4+RA+) and memory (CD4+ RO+) T cells were treated with IL-12 plus IL-18 for 48 hours in the presence or absence of SB203580, and the cells were then used for intracellular staining for IFN-γ. One representative donor among three is shown here, and data from rest of the donors are shown in Tables 2 & 3.