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. 2011 May 10;6(5):e19503. doi: 10.1371/journal.pone.0019503

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So et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a–c) To confirm the expression levels of PcG-targeting microRNAs in early and late passage MSCs and 5-AzaC-treated MSCs, real-time qPCR analysis was performed. Relative expression levels of mature microRNA 200c and 214 in early and late passage (a) and 1, 3 and 7day, 5-AzaC-treated hUCB-MSCs (b) were visualized. Relative expression levels of precursor microRNA 200c and 214 in 1–3 day, 5-AzaC-treated hUCB-MSCs were visualized (c). (d–e) miR200c and miR-214 inhibition and overexpression studies were performed. (d) Overexpression of both miRNAs induced cellular senescence of hUCB-MSCs, as shown by SA β-gal staining. (e) After transfection of anti- and mature-miRNA oligonucleotides, the expression levels of each miRNA and EZH2 and BMI1 were evaluated by real-time qPCR.