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. 2011 May 10;6(5):e19772. doi: 10.1371/journal.pone.0019772

Figure 2. Cre-recombinase is predominantly expressed in oligodendrocytes of P28 Plp-Cre mouse spinal cord.

Figure 2

A–I. Ventral white matter regions were double-stained with antibodies specific to CC-1 as a marker for mature oligodendrocytes (green), Cre (red) and counterstained with DAPI. Cre was not detected in WT spinal cord sections (B). Sections from transgenic mice of both F633 (D–F) and F627 (G–I) lines exhibited a similar pattern of Cre expression. High levels of Cre recombinase protein was detected in the CC1-positive oligodendrocytes. J. Ventral white matter region stained for GFAP (green) as a marker for astrocytes, Cre (red) and counterstained with DAPI. A small number of Cre-positive cells co-labeled with GFAP (arrow). K. Ventral white matter region stained for Ng2 (green) as a marker for OPCs, Cre (red) and counterstained with DAPI. A small number of Cre-positive cells co-labeled with Ng2 (arrow). L. Ventral grey matter region stained for NeuN (green) as a neuronal marker, Cre (red) and counterstained with DAPI. There were no NeuN positive cells that co-labeled with Cre. Scale bar  = 20 µm.