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. 2011 May 10;6(5):e19772. doi: 10.1371/journal.pone.0019772

Figure 5. Cre-recombinase is expressed both in and outside the oligodendrocyte lineage in the cerebellum of P4 Plp-Cre mice.

Figure 5

A–D, G–J. Deep cerebellar white matter regions from WT and Plp-Cre line 627 mice were double-stained with antibodies specific to Cre (red) and either CC-1 (green) as a marker for mature oligodendrocytes (A–B, G–H), or Ng2 (green) as a marker for OPCs (C–D, I–J) and counterstained with DAPI. Cre was not detected in WT sections (A–D). A large percentage of Cre-positive cells within the region co-stained for CC-1 and Ng2. E–F, K–L. Cerebellar sections of the developing granular layer from WT and Plp-Cre line 627 mice were double-stained with antibodies specific to Cre (red), the neuronal marker NeuN (green) and counterstained with DAPI. Cre was not detected in WT sections (E–F). Many Cre-positive cells co-stained for NeuN. Examples of Cre-positive cells co-labeling with neuronal or glial markers are denoted by arrows. Scale bar = 20 µm.