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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Feb 26;1808(7):1790–1796. doi: 10.1016/j.bbamem.2011.02.004

Figure 2. Substitution of Q713 with an amino acid that eliminates the interaction of the amide group (ε-oxygen) of glutamine residue in Q-loop with Mg++ cofactor significantly reduced Mg·ATP binding at NBD1.

Figure 2

Photolabeling experiments were carried out in the presence of 10 μM [γ-32P]-8-N3ATP on ice for 10 minutes. Molecular weight markers (kDa) are indicated on the left. NH and CH on the right indicate the 32P-labeled N-half and C-half of MRP1 protein.