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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Br J Haematol. 2011 Apr 15;153(5):589–598. doi: 10.1111/j.1365-2141.2011.08663.x

Fig 4. ETV6-LYN directly phosphorylates STAT5.

Fig 4

(A) In vitro kinase assays using ETV6-LYN and STAT5 immunoprecipitates. ETV6-LYN and ETV6-LYN KD were immunoprecipitated from BaF3/ETV6-LYN cultured without IL-3 and BaF3/ETV6-LYN KD cells cultured with IL-3, respectively, using an anti-ETV6 antibody. STAT5 was immunoprecipitated from Ba/F3 cells deprived of IL-3 for 12 h using an anti-STAT5B antibody. The in vitro kinase assay was performed under the indicated conditions. The proteins were then detected by Western blot analysis using anti-phosphotyrosine and anti-ETV6 antibodies. (B) In vitro kinase assays using ETV6-LYN and GST-STAT5A. GST-STAT5A and in vitro-translated ETV6-LYN and ETV6-LYN KD proteins were subjected to in vitro kinase assay under the indicated conditions. The proteins were then detected by Western blot analysis using anti-phosphotyrosine and anti-ETV6 antibodies.