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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Microbiol. 2011 Mar 30;80(4):1075–1087. doi: 10.1111/j.1365-2958.2011.07631.x

Fig. 2. MamE protease domain is essential for crystal maturation.

Fig. 2

A. Crystal size distribution of ΔR9ΔmamE complemented with wildtype, protease inactive (mamEP), and heme-binding deficient mamE (mamEC2). Crystal size is plotted as the percent of the total number of crystals that fall into each 5nm bin. n>500 crystals were measured for each strain from two independent ΔR9ΔmamE/mamE, three independent ΔR9ΔmamE/mamEP, and four independent ΔR9ΔmamE/mamEC2 cultures.

B. Representative TEM images of crystals for each of the three complemented strains. >100 fields were visualized by TEM from >5 independent cultures.