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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Microbiol. 2011 Mar 16;80(4):900–918. doi: 10.1111/j.1365-2958.2011.07619.x

Fig. 2. Surface accessibility of OmpATb in M. smegmatis by whole cell ELISA.

Fig. 2

Surface-accessible OmpATb was probed for on whole cells of M. smegmatis ML16 using a rabbit OmpATb-specific antiserum. Binding of a secondary alkaline phosphatase conjugate antibody was followed by addition of pNPP and subsequent absorbance readings at 405 nm. From left to right, 1st bar: M. smegmatis ML16 (ΔmspA ΔmspC ΔmspD) with the empty vector control pMS2; 2nd bar: ML16/pML2334; 3rd bar: ML16/pML588; 4th bar: ML16/pML003; 5th bar: ML16/pML1450. Data were obtained from four replicates and error bars represent standard errors.