Table 2. Plasmids used in this work.
Up- and downstream homologous sequences of genes are subscripted as up and down. “Origin” denotes origin of replication. The annotations HygR and KmR indicate that the plasmid confers resistance to hygromycin and kanamycin, respectively.
| Plasmid | Components and properties | Source or reference |
|---|---|---|
| pMS2 | ColE1 origin; PAL5000 origin; HygR; 5229 bp | (Kaps et al., 2001) |
| pMN016 | psmyc-mspA; ColE1 origin; PAL5000 origin; HygR; 6164 bp | (Stephan et al., 2005) |
| pMN013 | pimyc-mspA; ColE1 origin; PAL5000 origin; HygR; 6000 bp | (Mailaender et al., 2004) |
| pCV125 | ColE1 origin; KmR; L5 attP; L5 integrase; 8261 bp | (Steyn et al., 2003) |
| pML003 | psmyc-ompATb; ColE1 origin; PAL5000 origin; HygR; 6534 bp | (Song et al., 2008) |
| pML588 | pimyc-ompATb; ColE1 origin; PAL5000 origin; HygR; 6357 bp | (Song et al., 2008) |
| pML763 | pnative-ompATb-rv0900-rv0901; ColE1 origin; PAL5000 origin; HygR; 8023 bp | this study |
| pML1450 | psmyc-ompATb-rv0900-rv0901; ColE1 origin; PAL5000 origin; HygR; 7308 bp | this study |
| pML2334 | psmyc-ompATb73-326; ColE1 origin; PAL5000 origin; HygR; 6292 bp | this study |
| pML759 | ColE1 origin; pimyc-ompATb; KmR; L5 attP; L5 integrase; 9457 bp | this study |
| pML564 | ColE1 origin; PAL5000 origin; HygR; sacB; ompATbup, ompATbdown; 10630 bp | this study |
| pML591 | f1 origin; pT7-ompATbHis; KmR; 6129 bp | this study |