Figure 7.
Knockdown of Nogo-A prevented H-R-induced ROS generation and release of cytochrome c. A) Representative images of DCF fluorescence in NRVMs infected with shScr or shN adenovirus and subjected to normoxia or H-R. Scale bar: 10μM. B) Quantitative comparison of DCF fluorescence between NRVMs infected with Ad-shScr (open bars) or Ad-shN (closed bars) and subjected to normoxia or H-R. Data shown are the means ± SEM. *p < 0.01; **p < 0.001 (n=5); One-way ANOVA with Tukey’s post hoc analysis. C) Immunoblot analysis of cytosolic cytochrome c expression in NRVMs infected with shScr or shN adenovirus and subjected to normoxia or H-R.