Table 1.

NMR Spectroscopic Data for Ansalactam A (4) (methanol-d₄)^a

No.	δC, mult.b	δH (J in Hz)	COSY	HMBC
1	194.7, C
2	69.0, C
3	51.6, CH2	3.38, d (14.0) 2.92, d, (14.0)		1, 2, 4, 10, 21
4	195.8, C
5	129.8, C
6	161.7, C
7	134.9, C
8	133.4, CH	7.73, s		1, 6, 7, 9, 10
9	128.1, C
10	134.1, C
11	202.5, C
12	138.7, C
13	138.4, CH	6.91, s		11, 12-Me, 14-Me
14	135.4, C
15	144.9, CH	5.62, d (4.0)	16	13, 14-Me, 16-Me, 17
16	33.4, CH	3.17, m	15, 17	16-Me,
17	149.5, CH	6.19, d (6.6)	16	15, 16, 18-Me, 19
18	136.8, C
19	203.0, C
20	39.8, CH	3.24, dq (6.8, 2.4)	20-Me, 21	2, 19
21	58.4, CH	2.43, dd (10.1, 2.4)	20, 22	1, 2, 3, 4, 19
22	42.3, CH	3.70, br s	21, 24
23	183.8, C
24	41.3, CH2	1.52, m, 1.63, m	22, 25	23, 25, 26, 27
25	27.2, CH	1.97, m	24, 26, 27	22, 24, 26, 27
26	23.7, CH3	0.95, d (6.8)	25	24, 25, 27
27	22.5, CH3	0.92, d (6.8)	25	24, 25, 26
7-Me	16.9, CH3	2.31, s		6, 7, 8
12-Me	12.8, CH3	2.27, s		11, 13
14-Me	23.7, CH3	2.18, s		13, 15
16-Me	20.3, CH3	1.05, d (6.8)	16	15, 16, 17
18-Me	12.4, CH3	1.19, s		17, 19
20-Me	22.1, CH3	1.26, d (6.8)	20	19, 20, 21

^a600 MHz for ¹H NMR and 75 MHz for ¹³C NMR.

^bNumbers of attached protons were determined by analysis of 2D DEPT spectra.