A. Anti-BacM immunoblot of whole cell protein from M. xanthus, S. aurantiaca and P. mirabilis obtained by immediate freezing of cells followed by boiling in Laemmli buffer.
B. Anti-BacM immunofluorescence micrographs of S. aurantiaca cells from cultures with ODs of 0.10 (i), or 0.85 (ii) or after 7 h 100 µM cephalexin (iii). Pre-immune serum control of OD 0.85 cells (iv). Bar represents 2 µm.
C. Alignment of BacM with its putative orthologues from S. aurantiaca, BacMSaur, and from P. mirabilis, CcmA. Alternative names are given in parentheses. The N-terminal residues of the shorter forms of BacM (Ser27) and of CcmA (M59, according to Hay et al., 1999) are highlighted bold and underlined. Residues are highlighted grey if identical in at least two of the sequences.