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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Genes Brain Behav. 2011 Feb 10;10(4):392–403. doi: 10.1111/j.1601-183X.2011.00678.x

Figure 3. Rasgrf1 is expressed and imprinted in neonatal null olfactory bulb.

Figure 3

(a) Rasgrf1 transcript was amplified by RT-PCR in the olfactory bulbs and the remainder of the brain in null and biallelic animals using RT-PCR. Six mice were used for each assay. (b) Imprinted expression was assayed by RT-PCR followed by AciI digestion of the PCR products generated from tissues of progeny from reciprocal crosses between C57BL/6J (B6) and FVB/NJ (FVB) parents. Maternal strain is shown first. FVB expression generates 226bp and 130bp bands; B6 expression generates 210bp and 146bp bands. Digestion of amplicons (lanes labeled “C”) produced exclusively paternal banding patterns. Two to four mice were used for each test (lane pairs labeled “1” and “2” contain results from two different mice for each tissue type from each cross). C: cut/digested; U: undigested PCR products. Digest control lanes show transcript from each of the parent strains (F: FVB; B: B6).