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. Author manuscript; available in PMC: 2012 May 10.
Published in final edited form as: J Am Chem Soc. 2011 Apr 14;133(18):6968–6977. doi: 10.1021/ja107052p

Figure 3.

Figure 3

UV/visible absorption spectra of adenosylcobalamin in free solution, and in EAL in the holo-enzyme and ternary complex. The spectra were collected at 230 K for samples in the 50% dimethylsulfoxide/water cryosolvent system. (A) AdoCbl in EAL with bound (S)-2-amino-propanol substrate (ternary complex). (B) AdoCbl in EAL (holoenzyme). (C) AdoCbl in solution. Concentrations were as follows: 60 μM EAL, 30 μM AdoCbl, 5 mM (S)-2-amino-propanol.