Table 1.

Relative cob(II)alamin photoproduct amplitudes, limits on quantum yield and cage escape rate constant values, and observed recombination decay rate constants for cage escape populations at 240 K, following photolysis of AdoCbl with a saturating laser flash, in holo-EAL, holo-EAL with bound substrate, (S)-2-amino-propanol (ternary complext), and holo-EAL with bound substrate analog, (S)-1-amino-2-propanol. Standard deviations for determinations on at least three separate samples are shown in parentheses.

Condition	Pi	Arela	ϕ ib	kce,ic (s−1)	kdecay,i (s−1)
EAL•AdoCbl	P f	5.7 (1.1) ×10−1	<6×10−3	<5.8×105	5.0 (1.4) ×101
	P s	3.3 (0.6) ×10−1	<3×10−3	<2.9×105	4.3 (1.3) ×100
	P c	1.1 (0.7) ×10−1	<1×10−3	<9.6×104	-
EAL•AdoCbl	P f	6.1 (0.5) ×10−1	<6×10−3	<5.8×105	6.0 (1.4) ×101
•Substrate	P s	3.0 (0.3) ×10−1	<3×10−3	<2.9×105	4.9 (2.0) ×100
	P c	0.9 (0.5) ×10−1	<1×10−3	<9.6×104	-
EAL•AdoCbl	P f	5.7 (0.4)×10−1	<6×10−3	<5.8×105	6.0 (2.1) ×101
•Inhibitor	P s	3.4 (0.7) ×10−1	<3×10−3	<2.9×105	4.0 (1.9) ×100
	P c	0.9 (0.5) ×10−1	<1×10−2	<9.6×104	-

^aNormalized relative amplitude at 10⁻⁶ s, obtained by using saturating laser flash (10 mJ).

^bUpper limit on quantum yield, obained from relative amplitude and upper limit on absolute quantum yield at 10⁻⁶ s of <1×10⁻².

^cUpper limit on cage escape rate constant, obtained by using k_gr=9.5×10⁷ s⁻¹.